Invitrogen cell culture manual

Initiate cell culture from frozen stock The vial of S2 cells supplied contains ~1 × cells. Upon thawing, cells should have a viability of 60–70%. Once the culture is established, cell viability should be 95%. 1. Remove the vial of cells from liquid nitrogen and thaw quickly at 30°C. cell culture Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds . 3. Transfer cell suspension to a sterile mL centrifuge tube. 4. Determine the viable cell density and percent viability using a Countess ® Automated Cell Counter (similar automated or manual methods may be used) and calculate the required volume of Recovery ™ Cell Culture Freezing Medium to give a final cell density of 1 × 6. to 1 × File Size: KB.

The Invitrogen™ Countess™ II Automated Cell Counter uses trypan blue staining combined with an autofocus mechanism and sophisticated image analysis algorithm to obtain accurate cell and viability counts in as little as 10 seconds. Accurate—eliminate the subjectivity of manual cell counting and minimize user-to-user variability. Gibco Cell Culture Basics; Protein Methods Library; Supplemental Protocols; Newsletters Journals; Training Services; Events; Popular Tools Calculators. Lab Apps for Mobile Devices; Oligos Tools Utilities Hub; Tm Calculator for PCR; Molecular Biology Tools; Gibco Media Formulation Tool; Cell Culture Useful Numbers; Fluorescence. Initiating Cell Culture from Frozen Stock The following protocol is designed to help you initiate a cell culture from a frozen stock. The vial of S2 cells supplied contains ~1 x cells. Upon thawing, cells should have a viability of %. Once the culture is established, cell viability should be 95%. 1.

Gibco Cell Culture Basics; Protein Methods Library; Supplemental Protocols; Newsletters Journals; Training Services; Events; Popular Tools Calculators. Lab Apps for Mobile Devices; Oligos Tools Utilities Hub; Tm Calculator for PCR; Molecular Biology Tools; Gibco Media Formulation Tool; Cell Culture Useful Numbers; Fluorescence. Cell Culture Basics Companion Handbook is a supplement to the Cell Culture Basics instructional videos available online at www.doorway.ru The handbook and videos are intended as an introduction to cell culture basics, covering topics such as getting familiar with the requirements of a laboratory dedicated to cell. Invitrogen™ MagniSort™ Magnet. Designed to be used with 12 × 75mm round bottom polystyrene tubes (5mL), and can be used to magnetically separate up to 2 × 10 8 cells. For detailed instructions, follow the protocols provided with each separation kit.